期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 40, 期 5, 页码 1286-1290出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2006.09.015
关键词
bioreactor; laccase; orange peelings; solid-state fermentation; Trametes hirsuta
The present work focuses on the obtaining of high laccase activity levels by optimising several variables affecting laccase production under solid-state conditions. Thus, the effect of the addition of laccase-inducing compounds (syringaldazine, copper sulphate) and the amount of support utilised on laccase activity was studied. The highest activities (31,786 U/L) were obtained operating at the following conditions: 2.5 g ground orange peelings and 5 mM copper sulphate. The process was also scaled to 250 mL fixed-bed and 1.8 L tray reactors. The former produced maximal activities of about 3000 U/L, whereas the latter exhibited much higher values of around 12,000 U/L. To our knowledge, these activities are higher than those reported to date at bioreactor scale operating with a wild organism. (c) 2006 Elsevier Inc. All rights reserved.
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