期刊
FEBS LETTERS
卷 581, 期 7, 页码 1261-1268出版社
WILEY
DOI: 10.1016/j.febslet.2007.02.036
关键词
alpha-amylase; alpha-xylosidase; glycoside hydrolase family 13; glycoside hydrolase family 31; structural alignment; remote homology; evolutionary tree
Although both the alpha-amylase super-family, i.e. the glycoside hydrolase (GH) clan GH-H (the GH families 13, 70 and 77), and family GH31 share some characteristics, their different catalytic machinery prevents classification of GH31 in clan GH-H. A significant but remote evolutionary relatedness is, however, proposed for clan GH-H with GH31. A sequence alignment, based on the idea that residues equivalent in the primordial catalytic GH-H/GH31 (beta/alpha)(8)-barrel may not be found in the present-day GH-H and GH31 structures at strictly equivalent positions, shows remote sequence homologies covering beta 3, beta 4, beta 7 and beta 8 of the GH-H and GH31 (beta/alpha)(8)-barrels. Structure comparison of GH13 alpha-amylase and GH31 alpha-xylosidase guided alignment of GH-H and GH31 members for construction of evolutionary trees. The closest sequence relationship displayed by GH31 is to GH77 of clan GH-H. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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