PKCζII is a target for degradation through the tumour suppressor protein pVHL

PKC zeta II is a rapidly degraded variant of PKC zeta that suppresses epithelial cell polarisation. It is shown here that PKC zeta II is a target for the E3 ligase and tumour suppressor Von Hippel-Lindau protein (pVHL). Deletion studies demonstrate that the C-terminal region is required for the pVHL and proteasome dependent turnover of PKC zeta II, however it is the N-terminal PB1 domain of PKC zeta II that is required for pVHL complex formation. Reciprocal deletion studies define the pVHL effector domain as the dominant PKC zeta II binding site. The results indicate that pVHL recruits PKC zeta II via its PB1 domain and causes ubiquitination and degradation via the distal C-terminus of PKC zeta II. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.