期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 55, 期 7, 页码 2532-2535出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf063156c
关键词
silibinin; silipide; silybin; IDB 1016; HPLC; human plasma; milk thistle; Silybum marianum
资金
- MRC [G0100874] Funding Source: UKRI
- Medical Research Council [G0100874] Funding Source: researchfish
- Medical Research Council [G0100874] Funding Source: Medline
Silibinin has recently received attention as a potential cancer chemopreventive agent because of its antiproliferative and anticarcinogenic effects. A simple and specific reversed-phase high-performance liquid chromatography method was developed and validated for the quantitation of silibinin in human plasma. Sample preparation involved simple protein precipitation, and separation was achieved on a Waters Atlantis C-18 column with flow rate of 1.0 mL/min at 40 degrees C and UV detection at 290 nm. Silibinin was detected as two peaks corresponding to trans-diastereoisomers. The peak area was linear over the investigated concentration range (0-5000 ng/mL). The limits of detection were 2 and 1 ng/mL for the two diastereoisomers (d1 and d2), with a recovery of 53-58%. This method was utilized to detect silibinin in plasma of colorectal patients after 7 days of treatment with silipide (silibinin formulated with phosphatidyl choline).
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