期刊
CARBOHYDRATE POLYMERS
卷 68, 期 3, 页码 433-446出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2006.11.012
关键词
potato amylopectin starch; amylopectin structure; cluster structure; cluster interconnection; alpha-amylolysis
The cluster structure of amylose-free potato starch was investigated in detail. Groups of clusters (structural domains) were produced by alpha-amylolysis and size-fractionated by methanol precipitation. The domains were then further hydrolysed with alpha-amylase until free clusters were released. In the form of phi,beta-limit dextrins the clusters possessed mainly DP around 70 and 50-55, but a minor group of very small clusters were of DP 25. In order to release the clusters, the alpha-amylase hydrolysed long B-chains into shorter B1b-chains. In the initial stages of hydrolysis small, branched fragments were released, possibly from branched structures outside the clusters. In domain structures composed of two clusters a B2-chain was involved in their interconnection, but when the number of cluster increased, the number of long B2- and B3-chains was apparently higher than predicted from the generally accepted cluster model. The two-directional backbone model offered a possible solution for the interconnection of groups involving several clusters. (c) 2006 Elsevier Ltd. All rights reserved.
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