Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances

To simplify the molecular detection of micro-organisms, we evaluated the tolerance of loop-mediated isothermal amplification (LAMP) to a culture medium and some biological substances. The sensitivity of LAMP was less affected by the various components of the clinical samples than was polymerase chain reaction (PCR); therefore, DNA purification from samples could be omitted. (c) 2006 Elsevier B.V. All rights reserved.