期刊
MACROMOLECULAR BIOSCIENCE
卷 7, 期 4, 页码 435-439出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200700032
关键词
biosensors; chitosan; immobilized enzymes; polysaccharides
Chitosan was chemically branched with ferrocene moieties and further used as a support for the immobilization of horseradish peroxidase on a glassy carbon electrode. The reagentless biosensor device showed a linear amperometric response toward hydrogen peroxide concentrations between 35 X 10(-6) M and 2.0 x 10(-3) M. The biosensor reached 95% of the steady-state current in about 20 s and its sensitivity was 28.4 x 10(-3) mu A.M-1. The enzyme electrode retained 94% of its initial activity after 2 weeks of storage at 4 degrees C in 50 x 10(-3) M sodium phosphate buffer at pH 7.0.
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