On-line liquid chromatography-electrospray ionisation mass spectrometry for κ-carrageenan oligosaccharides with a porous graphitic carbon column

Enzymatically digested oligosaccharides of K-carrageenans were separated on a porous graphitic carbon (PGC) column and characterised on-line by electrospray ionisation mass spectrometry (ESI-MS). Two different developing ions were applied. Among them ammonium hydrogencarbonate showed more eluting power as it should on normal anion-exchange stationary phases. The oligosaccharides were detected by ESI-MS as fully deprotonated oligosaccharides. (c) 2007 Elsevier B.V. All rights reserved.