期刊
BIOSENSORS & BIOELECTRONICS
卷 22, 期 9-10, 页码 2025-2031出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2006.08.040
关键词
DNA sensor; label-free detection; DNA-modified polythiophene; electrochemical impedance spectroscopy; controlled delivery
A conducting polymer sensor for direct label-free DNA detection based on a polythiophene bearing an electroactive linker group is investigated. DNA hybridization is studied by electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance (QCM) techniques. Modelling of DNA hybridization by EIS measurements exhibits the contribution of nucleic acid to a superficial p-doping process. A 675-mer single-stranded DNA is produced using asymmetric PCR from a DNA sequence of a transposable element mariner and hybridized to the previously immobilized probe. Electrochemical stimulus leads to the release on demand of DNA fragments and the amount delivery permits to do PCR amplification. (c) 2006 Elsevier B.V. All rights reserved.
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