期刊
BIOSENSORS & BIOELECTRONICS
卷 22, 期 9-10, 页码 2377-2381出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2006.09.038
关键词
electrochemical biosensors; single-walled carbon nanotubes; arrayed electrodes; prostate-specific antigen; immunosensor
We have fabricated a label-free electrochemical immunosensor using microelectrode arrays modified with single-walled carbon nanowbes (SWNTs). Label-free detection of a cancer marker, total prostate-specific antigen (T-PSA), was carried out using differential pulse voltammetry (DPV). The current signals, derived from the oxidation of tyrosine (Tyr), and tryptophan (Trp) residues, increased with the interaction between T-PSA on T-PSA-mAb covalently immobilized on SWNTs. The selectivity of our biosensor was challenged using bovine serum albumin (BSA) as the target protein. The detection limit for T-PSA was determined as 0.25 ng/mL. Since the cut-off limit of T-PSA between prostate hyperplasia and cancer is 4 ng/mL, the performance of our label-free electrochemical immunosensor seems promising for further clinical applications. (c) 2006 Elsevier B.V. All rights reserved.
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