DNA-binding and photocleavage studies of mixed polypyridyl ruthenium(II) complexes with calf thymus DNA

New mixed polypyridyl {NMIP=2'-(2-nitro-3,4-methylenedioxyphenyl)imidazo-[4',5'-f][1,10]-phenanthroline, dmb=4,4'-dimethyl-2,2'-bipyridine, bpy = 2,2'-bipyridine} ruthenium(II) complexes [Ru(dmb)(2)(NMIP)](2+) (1) and [R-a(bpy)(2)(NMIP)](2+) (2) have been synthesized and characterized. The binding of these complexes to calf thymus DNA (CT-DNA) has been investigated with spectroscopic methods, viscosity and electrophoresis measurements. The experimental results indicate that both complexes could bind to DNA via partial intercalation from the minor/major groove. In addition, both complexes have been found to promote the single-stranded cleavage of plasmid pBR 322 DNA upon irradiation. Under comparable experimental conditions compared with [Ru(phen)(2)(NMIP)](2+), during the course of the dialysis at intervals of time, the CD signals of both complexes started from none, increased to the maximum magnitude, then no longer changed, and the activity of effective DNA cleavage dependence upon concentration degree lies in the following order: [Ru(phen)(2)NMIP](2+) > complex 2 > complex 1. (c) 2006 Elsevier B.V. All rights reserved.