期刊
BLOOD
卷 109, 期 9, 页码 3906-3914出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2006-07-036335
关键词
-
类别
资金
- Intramural NIH HHS Funding Source: Medline
- NCI NIH HHS [P01 CA 101937, P01 CA101937] Funding Source: Medline
- NHLBI NIH HHS [T32 HL007088, T32 HL 07088-31A1] Funding Source: Medline
Expression of the constitutively activated TEL/PDGF beta R fusion protein is associated with the t(5;12)(q33;pl3) chromosomal translocation found in a subset of patients with chronic myelomonocytic leukemia. TEL/PDGF beta R activates multiple signal transduction pathways in cell-culture systems, and expression of the TEL-PDGFRB fusion gene induces myeloproliferative disease (MPD) in mice. We used gene-targeted mice to characterize the contribution of signal transducer and activator of transcription (Stat) and Src family genes to TEL-PDGFRB-mediated transformation in methylcellulose colony and murine bone marrow transduction/ transplantation assays. Fetal liver hematopoietic stem and progenitor cells harboring targeted deletion of both Stat5a and Stat5b (Staffab(null/null)) genes were refractory to transformation by TELPDGFRB in methylcellulose colony assays. Notably, these cell populations were maintained in Stat5ab(null/null) fetal livers and succumbed to transformation by c-Myc. Surprisingly, targeted disruption of either Stat5a or Stat5b alone also impaired TEL-PDGFRB-mediated transformation. Survival of TFIGFP -> Stat5a(-/-) and TPIGFP -> Stat5a(+/-) mice was significantly prolonged, demonstrating significant sensitivity of TEL-PDGFRB-induced MPD to the dosage of Stat5a. TEL-PDGFRB-mediated MPD was incompletely penetrant in TFIGFP -> Stat5b(-/-) mice. In contrast, Src family kinases Lyn, Hck, and Fgr and the Stat family member Stat1 were dispensable for TEL-PDGFRB disease. Together, these data demonstrate that Stat5a and Stat5b are dose-limiting mediators of TEL-PDGFRB-induced myeloproliferation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据