期刊
METABOLIC ENGINEERING
卷 9, 期 3, 页码 258-267出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2006.12.002
关键词
sigma factor engineering; ethanol tolerance; metabolic engineering; directed evolution; lycopene production
It is now generally accepted that most cellular phenotypes are affected by many genes. As a result, engineering a desired phenotype would be facilitated enormously by simultaneous multiple gene modification, yet the capacity to introduce such modifications is very limited. Here, we demonstrate that the components of global cellular transcription machinery (specifically, a 70) can be engineered to allow for global perturbations of the transcriptome, which can help unlock complex phenotypes. Results from three distinct phenotypes (ethanol tolerance, metabolite overproduction, and multiple phenotypes) are provided as proof- of-concept. In each case, the tool of global transcription machinery engineering (gTME) outperformed traditional approaches by quickly and more effectively optimizing phenotypes. (c) 2007 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据