期刊
NATURE CELL BIOLOGY
卷 9, 期 5, 页码 516-U56出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb1576
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资金
- NCRR NIH HHS [P41 RR001219, P41 RR01219] Funding Source: Medline
- NIGMS NIH HHS [R01 GM066270, GM59363, R01 GM06627, R01 GM059363, R01 GM059363-09, R01 GM066270-05A1] Funding Source: Medline
Intricate interactions between kinetochores and microtubules are essential for the proper distribution of chromosomes during mitosis. A crucial long-standing question is how vertebrate kinetochores generate chromosome motion while maintaining attachments to the dynamic plus ends of the multiple kinetochore MTs (kMTs) in a kinetochore fibre. Here, we demonstrate that individual kMTs in PtK1 cells are attached to the kinetochore outer plate by several fibres that either embed the microtubule plus- end tips in a radial mesh, or extend out from the outer plate to bind microtubule walls. The extended fibres also interact with the walls of nearby microtubules that are not part of the kinetochore fibre. These structural data, in combination with other recent reports, support a network model of kMT attachment wherein the fibrous network in the unbound outer plate, including the Hec1-Ndc80 complex, dissociates and rearranges to form kMT attachments.
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