期刊
HUMAN REPRODUCTION
卷 22, 期 5, 页码 1247-1252出版社
OXFORD UNIV PRESS
DOI: 10.1093/humrep/del519
关键词
non-human primate; granulosa cell; proliferation; luteinization; epidermal growth factor
资金
- NCRR NIH HHS [RR00169, RR13439] Funding Source: Medline
- NICHD NIH HHS [HD047964, HD043358] Funding Source: Medline
BACKGROUND: This study seeks to clarify cell cycle dynamics of granulosa cells following hCG and elucidate theexpression of epidermal growth factor (EGF)-like ligands during luteinization. METHODS: Granulosa cells were obtained from rhesus macaques undergoing controlled ovarian stimulation protocols before or after an ovulatory hCG bolus. Cell cycle characteristics were determined by flow cytometry and levels of EGF receptor (EGFR), amphiregulin (AREG), epiregulin (EREG) and betacellulin (BTC) mRNAs were measured by real-time RT-PCR. RESULTS: The proportion of cells in S-phase was 7.5% prior to hCG and did not decline until 24 h after hCG (3.1%). EGFR protein and BTC mRNA did not change following hCG, whereas AREG and EREG mRNA increased starting at 3 and 12 h post-hCG, respectively, and remained elevated thereafter. CONCLUSIONS: Cell cycle transit of macaque granulosa cells does not change until 24 h after an ovulatory stimulus, whereas the EGF-like ligands EREG and AREG are increased rapidly. This suggests that luteinizing granulosa cells are refractory to mitogenic stimulation by EGFR ligands.
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