期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 119, 期 5, 页码 1210-1217出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2007.03.006
关键词
atopic dermatitis; psoriasis; myeloid DCs; inflammatory dendritic epidermal cells; plasmacytoid DCs; TIP-DCs
资金
- NCRR NIH HHS [UL1RR024143] Funding Source: Medline
- NIAMS NIH HHS [K23 AR052404-01A1] Funding Source: Medline
Background: Atopic dermatitis (AD) and psoriasis represent contrasting poles of the T-H(1) versus T-H(2) paradigm. Both diseases have been associated with increased numbers of dendritic cells (DCs) in the skin, but the similarities and differences in DC populations need to be established. Objective: We aimed to characterize the specific DC subsets, as well as chemokine and cytokine environment in chronic AD compared with psoriasis. Methods: Skin biopsies were obtained from patients with acute exacerbation of chronic AD (n = 18), psoriasis (n = 15), and healthy volunteers (n = 15) for microarray analysis, RT-PCR, immunohistochemistry, and double-label immunofluorescence. Results: Myeloid DCs upregulate CCL17 and CCL18 in AD, as opposed to TNF-a and inducible nitric oxide synthase (iNOS) in psoriasis. In our study, we identified cells phenotypically identical to the inflammatory dendritic epidermal cells in the dermis in both diseases, although to a lesser extent in psoriasis. We found substantially higher numbers of dermal CCL22 producing plasmacytoid DCs in AD. The thymic stromal lymphopoietin receptor showed significantly higher expression in AD, whereas the thymic stromal lymphopoietin ligand was upregulated more in psoriasis. Conclusion: There are major differences in myeloid and plasmacytoid subsets of cutaneous DCs and the chemokine/ cytokine environment between AD and psoriasis. Distinct subsets within the CD11c(+) population may influence polarization through the production of regulatory mediators, including NOS, TNF, CCL17, and CCL18. Plasmacytoid DCs may also influence TH2 polarization, having a more important role in AD than previously appreciated. Clinical implications: Dermal inflammatory dendritic cells in AD and TNF and iNOS-producing DCs in psoriasis, and/or their regulatory products, may be potential targets for future therapeutic interventions.
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