期刊
ELECTROCHEMISTRY COMMUNICATIONS
卷 9, 期 5, 页码 1086-1090出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2006.12.028
关键词
EcoRI endonuclease; DNA cleavage; ferrocene; site-specific; gold nanoparticle; electrochemical analysis
An electrochemical method was proposed to study DNA cleavage by EcoRI endonuclease using ferrocene as electrochemical indicator. Ferrocene was not covalently crosslinked to the DNA molecule but co-immobilized on the gold natioparticles with thiolated target DNA via Au-S bond. EcoRI-DNA interaction was monitored based on the alteration of ferrocene signal before and after digestion with EcoRI. Enzymatic cleavage specificity of EcoRI was investigated, and enzymatic cleavage at DNA-modified electrode was also monitored real-time. (c) 2006 Elsevier B.V. All rights reserved.
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