期刊
MICROELECTRONIC ENGINEERING
卷 84, 期 5-8, 页码 1702-1705出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mee.2007.01.267
关键词
micro-stereolithography; poly(propylene famarate) (PPF); diethyl fumarate (DEF); 3D scaffold fabrication; cell culture
Current studies on scaffold fabrication have focused on overcoming the limitations imposed by the mechanical properties of existing biodegradable materials and the irregular structures they produce. Recently, several promising biodegradable materials were introduced, including poly(propylene fumarate) (PPF). In addition, the development of micro-stereolithography allows the fabrication of free-form 3D microstructures by dividing a desired shape into several slices of a given thickness. This technology, however, requires a low-viscosity resin to fabricate fine structures, which excludes the use of PPF. To fabricate precise 3D scaffolds using micro-stereolithography, we created a system in which the viscosity of PPF was reduced by adding diethyl fumarate. The fabricated scaffold was sterilized, and fibroblasts in cell culture medium were seeded onto the structure. Cells were fixed and freeze-dried after 4, 7, and 28 days of culture. Under scanning electron microscopy, we observed that the cells were able to attach to the scaffold surface and grow. (c) 2007 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据