Amperometric glucose biosensing of gold nanoparticles and carbon nanotube multilayer membranes

A novel multilayer gold nanoparticles/multiwalled carbon nanotubes/glucose oxidase membrane was prepared by electrostatic assembly using positively charged poly(dimethyldiallylammonium chloride) to connect them layer by layer. ne modification process and membrane structures were characterized by atomic force microscopy, scanning electron microscopy and electrochemical methods. This membrane showed excellent electrocatalytic character for glucose biosensing at a relatively low potential (- 0.2 V). The K-m value of the immobilized glucose oxidase was 10.6 mM. This resulting sensor could detect glucose up to 9.0 mM with a detection limit of 128 mu M and showed excellent analytical performance.