期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 158, 期 2, 页码 196-204出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2006.10.030
关键词
electron microscopy; electron tomography; cryo-microscopy; noise reduction; image processing; automation
资金
- NIGMS NIH HHS [U54 GM646346, GM64473] Funding Source: Medline
A simple, fast and efficient noise-reduction protocol for three-dimensional electron tomographic reconstructions of biological material is presented. The approach is based on iterative application of median filtering and shows promise for automatic noise reduction as a preprocessor for automated data analysis tools which aim at segmentation, feature extraction and pattern recognition. The application of this algorithm produces encouraging results for a wide variety of experimental and synthetic electron tomographic reconstructions. (c) 2006 Elsevier Inc. All rights reserved.
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