期刊
CURRENT GENETICS
卷 51, 期 5, 页码 309-319出版社
SPRINGER
DOI: 10.1007/s00294-007-0127-0
关键词
Rnq1 [PIN+]; yeast; prion; [PSI+]; induction
资金
- NIGMS NIH HHS [R01 GM056350, R01 GM056350-12, GM 56350] Funding Source: Medline
Prions are viewed as enigmatic infectious entities whose genetic properties are enciphered solely in an array of self-propagating protein aggregate conformations. Rnq1, a yeast protein with yet unknown function, forms a prion named [PIN+] for its ability to facilitate the de novo induction of another prion, [PSI+]. Here we investigate a set of RNQ1 truncations that were designed to cover major Rnq1 sequence elements similar to those important for the propagation of other yeast prions: a region rich in asparagines and glutamines and several types of oligopeptide repeats. Proteins encoded by these RNQ1 truncations were tested for their ability to (a) join (decorate) pre-existing [PIN+] aggregates made of wild-type Rnq1 and (b) maintain the heritable aggregated state in the absence of wildtype RNQ1. While the possible involvement of particular sequence elements in the propagation of [PIN] is discussed, the major result is that the efficiency of transmission of [PIN+] from wild-type Rnq1 to a fragment decreased with the fragment's length.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据