期刊
ELECTROANALYSIS
卷 19, 期 9, 页码 1008-1014出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200603808
关键词
glucose sensor; zinc oxide; nanowire; apparent Michaelis constants
ZnO nanowire was tailored both physically and chemically to immobilize the enzyme glucose oxidase (GOD) for construction of a glucose sensor with high performance, which was ascribed to its high specific surface area and high isoelectric point value for efficient immobilization of high concentration of acidic enzymes and the mediating effect by the redox reaction of ZnO nanowires. The apparent Michaelis constants J(max), and K-M were adjusted in a large scope by tailoring the thickness of the GOD/ZnO nanowire layer and the enzyme load in the nanowired network. Thus, a variety of linear region, sensitivities and reaction rates of the sensor could be easily achieved. Moreover, the glucose OF sensor showed long term stability with the incorporation of the inorganic zinc oxide nanowire.
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