Simultaneous determination of tiopronin and D-penicillamine in human urine by liquid chromatography with ultraviolet detection

D-Penicillamine and tiopronin are drugs widely used for the treatment of many diseases. Because of the relatively high frequency of side effects to these compounds, some of which are dose-related, drug monitoring in urine samples during treatment is advisable. In this paper, we describe a simple method for the determination of tiopronin and D-penicillamine in human urine. The method was based on derivatization with 2chloro-1-methylquinolinium tetrafluoroborate followed by ion-pairing reversed-phase liquid chromatography separation and ultraviolet-absorbance detection. 2-S-quinolinium derivatives of thiols were detected at 355 nm. The derivatization was optimized in terms of pH and time of the reaction. Baseline separation was achieved on an analytical Zorbax SB C-18 (5 mu m, 150 mm x 4.6 mm) column with a mobile phase consisting of pH 2.0 0.09 mol L-1 trichloroacetic acid buffer (component A) and acetonitrile (component B) pumped at 1.0 mL min(-1). Gradient elution was used: 0-4 min, 12% B; 4-8 min, 12-40% B; 8-12 min, 40-12% B. The D-penicillamine and tiopronin standards added to the urine show that the response of the detector is linear within the range studied, from 1 to 200 mu mol L-1 urine. The imprecision ranges for tiopronin and D-penicillamine were within 1.61-8.24% and 2.92-10.60%, respectively. The analytical accuracy for determined compounds was from 97.24 to 109.39%. The lower limits of detection and quantitation were 0.5 mu mol L-1 and 1.0 mu mol L-1 urine, respectively. This method can be used for routine clinical monitoring of the title thiol-drugs. Cysteine can be measured concurrently, if needed. (c) 2007 Elsevier B.V. All rights reserved.