期刊
EMBO JOURNAL
卷 26, 期 9, 页码 2387-2399出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.emboj.7601671
关键词
enhancer; promoter; thymus; V beta 8.2
资金
- NIGMS NIH HHS [R01 GM059638-05, R01GM059638, R01 GM059638-06, R01 GM059638-07, R01 GM059638-08, R01 GM059638] Funding Source: Medline
Allelic exclusion operates in B and T lymphocytes to ensure clonal expression of antigen receptors after V(D) J recombination. Germline transcription, which proceeds V(D) J recombination, has been postulated to provide an instructive signal for allelic exclusion. Here, we use a genetic marker to track germline transcription from a V beta gene within the TCRb locus. We find that developing thymocytes exhibit uniformed, bi-allelic activation of the V beta gene before V-DJ recombination, a process subject to allelic exclusion. We further show that V-DJ rearrangement promotes activation rather than silencing of germline transcription from the remaining V beta genes on either the functionally or non- functionally rearranged chromosome. Results presented here suggest that germline transcription, although necessary for V(D) J recombination, is not sufficient to instruct allelic exclusion.
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