期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 282, 期 18, 页码 13585-13591出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M700463200
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资金
- NCI NIH HHS [CA67754, CA11227] Funding Source: Medline
- NIAID NIH HHS [AI47673, 5F31AI05891602] Funding Source: Medline
- NIGMS NIH HHS [GM77471, GM33063] Funding Source: Medline
- NINDS NIH HHS [NS047101] Funding Source: Medline
Facilitating the uptake of molecules into living cells is of substantial interest for basic research and drug delivery applications. Arginine-rich peptides have been shown to facilitate uptake of high molecular mass cargos into cells, but the mechanism of uptake is complex and may involve multiple receptors. In this report, we show that a derivative of the aminoglycoside antibiotic neomycin, in which all of the ammonium groups have been converted into guanidinium groups, can carry large (> 300 kDa) bioactive molecules across cell membranes. Delivery occurs at nanomolar transporter concentrations and under these conditions depends entirely on cell surface heparan sulfate proteoglycans. Conjugation of guanidinoneomycin to the plant toxin saporin, a ribosome-inactivating agent, results in proteoglycan-dependent cell toxicity. In contrast, an arginine-rich peptide shows both heparan sulfate-dependent and -independent cellular uptake. The high selectivity of guanidi-noneomycin for heparan sulfate suggests the possibility of exploiting differences in proteoglycan compositions to target delivery to different cell types.
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