期刊
BRAIN RESEARCH
卷 1145, 期 -, 页码 28-40出版社
ELSEVIER
DOI: 10.1016/j.brainres.2007.01.103
关键词
K-ATP; diazoxide; cyclic GMP; sensitization; action potential
资金
- NCRR NIH HHS [C06 RR015481, C06 RR015481-01] Funding Source: Medline
- NINDS NIH HHS [NS36956, R01 NS036956-04] Funding Source: Medline
Behavioral studies have shown that the hyperalgesia arising from inflammatory agents, such as prostaglandin E-2 (PGE(2)), can be antagonized by activators of the ATP-sensitive potassium current (KATP). This observation raises questions as to whether this suppression results from a direct action on sensory neurons and what are the cellular mechanisms giving rise to this inhibition. We found that small to medium diameter sensory neurons isolated from the L4-6 DRGs expressed the mRNAs for Kir6.1, Kir6.2, and SUR1. In perforated-patch clamp recordings from acutely dissociated sensory neurons from the young adult rat, exposure to 300 mu M diazoxide, a K-ATP channel agonist, significantly hyperpolarized the resting membrane potential, reduced the number of action potentials evoked by a ramp of depolarizing current, and increased the amplitude of inward K-ATP currents evoked by the voltage ramp. Similar results were obtained with the protonophore FCCP, which is known to reduce the levels of intracellular ATP and lead to the activation of K-ATP. Only a subpopulation of sensory neurons was sensitive to diazoxide whereas other neurons were unaffected. Treatment with 1 mu M PGE(2) significantly enhanced the excitability of these small to medium diameter capsaicin- sensitive sensory neurons; this enhancement was reversed by subsequent exposure to diazoxide in a subpopulation of neurons. Similar to diazoxide, exposure to 8-Br-cyclic GMP antagonized the PGE(2)-induced increase in excitability. The effects of 8-Br-cyclic GMP could be reversed by exposure to glibenclamide, an antagonist of K-ATP channels. As with diazoxide, only a subpopulation of sensory neurons were affected by 8-Br-cyclic GMP. These results demonstrate that activation of KATP can reverse the sensitization produced by PGE(2) and may be an important means to modulate the enhanced excitability that results from inflammatory or injury conditions. (c) 2007 Elsevier B.V. All rights reserved.
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