期刊
BIOSENSORS & BIOELECTRONICS
卷 22, 期 11, 页码 2516-2524出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2006.10.011
关键词
cholesterol; cholesterol oxidase (ChOx); surface plasmon resonance (SPR); poly-(3-hexylthiophene) (P3HT); electrochemical impedance; 1-fluoro-2-nitro-4-azidobenzene (FNAB)
Cholesterol oxidase (ChOx) has been covalently immobilized onto 1-fluoro-2-nitro-4-azidobenzene (FNAB) modified poly-(3-hexylthiophene) (P3HT) self-assembled monolayer (SAM) onto gold coated glass plates. These ChOx./FNAB/P3HT/Au bio-electrodes have been characterized using contact angle (CA) measurements, UV-vis spectroscopy, electrochemical impedance technique, cyclic voltammetric technique and atomic force microscopic (AFM) technique, respectively. The ChOx/FNAB/P3HT/Au bio-electrodes were utilized for the estimation of cholesterol concentration in standard solutions using surface plasmon resonance (SPR) technique. It is shown that this SPR biosensor has linearity from 50 to 500 mg/dl of cholesterol in solution with detection limit of 50 mg/dl, sensitivity of 1.04 m degrees/(mg dl), reusability of around 15 times and a shelf-life of about 10 weeks when stored at 4 degrees C. (c) 2006 Elsevier B.V. All rights reserved.
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