期刊
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
卷 67, 期 3, 页码 526-530出版社
WILEY
DOI: 10.1002/prot.21364
关键词
ensemble; distance; paramagnetic; relaxation; enhancement
资金
- NCRR NIH HHS [P20 RR 16448, P20 RR 16454-02] Funding Source: Medline
Paramagnetic relaxation enhancement (PRE) was used to identify a compact dynamic structure for the intrinsically unstructured transactivation domain of the tumor suppressor protein, p53. Our results show that p53 residues essential for binding to the ubiquitin ligase, MDM2, and the 70 kDa subunit of replication protein A, RPA70, are separated by an average distance of 10-15 angstrom. This result suggests that a more extended member of the ensemble must be populated prior to binding either MDM2 or RPA70. We also show that PRE can be used to detect intermolecular distances between p53 and RPA70.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据