期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 111, 期 3, 页码 667-670出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2007.01.017
关键词
Lonicera japonica; cyclooxygenase 2 (COX-2); transcription; expression; prostaglandin
The anti-inflammatory properties of aqueous extracts from Lonicera japonica (LJ) flower, an anti-inflammatory treatment in traditional Chinese medicine, were tested by radioimmunoassay of cyclooxygenase isoenzyme-generated prostaglandin E-2 (PGE(2)) synthesis as well as by Western and Northern blot analysis of COX-2 protein and mRNA expression, respectively. Boiled LJ aqueous extracts directly inhibited both COX-1 and COX-2 activity, while non-boiled extracts stimulated COX-1. Boiled LJ extracts also inhibited expression of IL-1 beta-induced COX-2 protein expression and suppressed its mRNA induction by IL-1 beta in A549 cells. Suppression of COX-2 mRNA induction required a significantly higher dose of aqueous extract than did suppression of protein expression, indicating that compounds in the extract act translationally or post-translationally at lower doses and transcriptionally or post-transcriptionally at higher doses. Direct inhibition of COX isoenzymes as well as down-regulation of COX-2 mRNA and protein may represent the mechanism by which this ancient herbal treatment decreases inflammation. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
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