期刊
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
卷 87, 期 2, 页码 130-136出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2007.03.006
关键词
bioluminescence; ATP; earthworms; luciferin; luciferase; enchytraeid
A homogeneous luciferin preparation has been obtained from the luminous soil enchytraeid Fridericia heliota, which has an ATP-dependent luminescent system. A procedure for luciferin purification without losing fractions of active luciferase has been developed. The luciferin specific activity is 4000 times increased; its UV absorption spectrum maximum is 294 nm with a local minimum at 262 nm. The luciferin of the enchytraeid F. heliota is significantly different from firefly luciferin, whose luminescent reaction also requires ATP, and it also appears to have no similarities to other known luciferins. (c) 2007 Elsevier B.V. All rights reserved.
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