Neuroprotective effect of crocin on substantia nigra in MPTP-induced Parkinson's disease model of mice

Parkinson's disease is caused by damage to substantia nigra dopaminergic neurons. Factors such as oxidative stress, inflammatory factors, and acetylcholinesterase activity may induce this disease. On the other hand, crocinone of the active ingredients of saffronhas anti-oxidant and anti-inflammatory properties. This study was performed to evaluate the protective effect of crocin to decrease dopaminergic neuron damage and Parkinson's disease complications induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). A set of 24 male BALB/c Mice were divided randomly into four groups: (1) MPTP group receiving 30mg/kg MPTP for 5days; (2) MPTP+crocin group receiving 30mg/kg MPTP for 5days and 30mg/kg crocin for 15days; (3) NS group receiving normal saline for 5days; and (4) NSIG group receiving normal saline intraperitoneally for 5days and also normal saline by gavage for 15days. After the treatment period, pole and hanging motor tests were performed in all groups. Then, the brains of all the animals were removed and fixed in formalin, prepared according to routine histologic methods and cut into sections of 5 mu m thickness. Prepared sections were stained by immunohistochemistry techniques and toluidine blue to detect tyrosine-hydroxylase (TH)-positive neurons and dark neurons, respectively. Finally, the mean number of these cells were calculated by stereological methods and compared with the statistical tests in different groups. The results showed a significant increase in the time taken for the animal to fall from the pole in the MPTP group in comparison with other groups (P<0.001). The time taken for them to stay on the wire in the hanging test decreased significantly in the MPTP group compared to the other groups (P<0.001).,while in the MPTP+crocin group, the time to falling decreased (P<0.05) and the time staying on the wire increased (P<0.001) compared to the MPTP group. The number of TH-positive neurons in the MPTP group also decreased significantly in comparison with saline and MPTP+crocin groups (P<0.001). The number of dark neuron sin the MPTP group increased significantly as compared with saline and the MPTP+Crocin groups (P<0.001). Our results showed that crocin improves MPTP-induced Parkinson's disease complications and decreases cell death in the substantia nigra.