Structural and morphological studies on poly(3-hydroxybutyrate acid) (PHB)/chitosan drug releasing microspheres prepared by both single and double emulsion processes

Drug releasing microspheres of poly(3-hydroxybutyric acid)/chitosan (PHB/CTS) with various compositions have been synthesized by both single and double emulsion methods, and collected by a freeze-drying process. In this study, gentamicin was used as an antibacterial medicine coated with PHB. The PHB/CTS microspheres of various compositions prepared by a single emulsion process (SEP) were identified as the major PHB phase together with a minor unknown Phase X by X-ray diffraction (XRD) and FT-IR. However, in the microspheres prepared using a double emulsion process (DEP) the dominant Phase was X and the minor phase was PHB. The size of the PHB/CTS rnicrospheres prepared by SEP increased with the PHB/CTS ratio from 1 mu m for 1 : 1 to 2 mu m for 5:1. However, the size of the PHB/CTS microspheres prepared by DEP decreased with the PHB/CTS ratio from 1 mu m for 1:1 to 800 nm for 5:1. (C) 2006 Elsevier B.V. All rights reserved.