期刊
CHROMATOGRAPHIA
卷 65, 期 11-12, 页码 701-706出版社
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-007-0204-z
关键词
column liquid chromatography; extraction and hydrolysis parameters; flavonoids; Hypericum perforatum
The optimum conditions for extraction of rutin and quercetin from Hypericum perforatum were investigated. The best efficiency of extraction was achieved with aqueous methanol 40-80% (v/v). For quercetin analysis as aglycone the effect of acid concentration and hydrolysis time on the extraction recovery were also studied. Hydrolysis for 5 min in the presence of 2.8 mol L-1 HCl as well as for 10 min with 1.1 mol L-1 HCl efficiently released quercetin from rutin. The content of quercetin-3-O-glycosides (rutin, hyperoside and quercetrin) and quercetin aglycone as well as chlorogenic and caffeic acids in H. perforatum leaves and flowers were determined by HPLC with photodiode-array detection and confirmed by electrospray mass spectrometry.
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