期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 378, 期 3, 页码 352-365出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2007.02.038
关键词
steroid estrogen; river water; wastewater effluent; LC-MS/MS; endocrine disruptors
River water and wastewater treatment plant (WWTP) effluents from metropolitan Taipei, Taiwan were tested for the presence of the pollutants estrone (E-1), estriol (E-3) 17 beta-estradiol (E-2) and 17 alpha-ethinylestradiol (EE2) using a new methodology that involves high-flow solid-phase extraction and liquid chromatography/tandem mass spectrometry. The method was also used to investigate the removal of the analytes by conventional drinking water treatment processes. Without adjusting the pH, we extracted 1-L samples with PolarPlus C-18 Speedisks under a flow rate exceeding 100 mL/min, in which six samples could be done simultaneously using an extraction station. The adsorbent was washed with 40% methanol/60% water and then eluted by 50% methanol/50% dichloromethane. The eluate was concentrated until almost dry and was reconstituted by 20 mu L of methanol. Quantitation was done by LC-MS/MS-negative electrospray ionization in the selected reaction monitoring mode with isotope-dilution techniques. The mobile phase was 10 mM N-methylmorpholine aqueous solution/acetonitrile with gradient elution. Mean recoveries of spiked Milli-Q water were 65-79% and precisions were within 220% of the tested concentrations (5.0-200 ng/L). The method was validated with spiked upstream river water; precisions were most within 10% of the tested concentrations (10- 100 ng/L) with most RSDs < 10%. LODs of the environmental matrixes were 0.78-7.65 ng/ L. A pre-filtration step before solid-phase extraction may significantly influence the measurement of E, and EE2 concentrations; disk overloading by water matrix may also impact analyte recoveries along with ion suppression. In the Taipei water study, the four steroid estrogenswere detectedin riversamples (ca. 15 ng/L for E-2 and EE2 and 35-45 ng/Lfor E-1 and E-3). Average levels of 19-26ng/L for E-1, E-2, and EE2 were detected inmost wastewater effluents, while only a single effluent sample contained E-3-The higher level in the river was likely caused by the discharge of untreated human and fanning waste into the water. In the drinking water treatment simulations, coagulation removed 20-50% of the estrogens. An increased dose of aluminum sulfate did not improve the performance. Despite the reactive phenolic moiety in the analytes, the steroids were decreased only 20-44% of the initial concentrations in pre- or post-chlorination. Rapid filtration, with crushed anthracite playing a major role, took out more than 84% of the estrogens. Except for E3, the whole procedure successfully removed most of the estrogens even if the initial concentration reached levels as high as 500 ng/L. (c) 2007 Elsevier B.V. All rights reserved.
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