期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
卷 147, 期 2, 页码 502-508出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpa.2007.01.026
关键词
Japanese seabass; Lateolabrax japonicus; exogenous enzymes; nitrogenous excretion; phosphorus excretion; growth; feeding utilization; feeding and nutrition
A feeding experiment was conducted to investigate the effects of exogenous enzymes (phytase, non-starch polysaccharide enzymes: WX and VP, supplied by DSM) on growth performance and excretion of Japanese seabass (Lateolabrax japonicus) (initial mass 6.26 +/- 0.10 g). A basal diet was used as a control that contained a mixed protein source, and lysine, methionine and valine were supplemented to simulate the essential amino acid pattern of fish meal. Four other diets were supplemented with 200 mg phytase (500 IU), 400 mg VP, 800 mg WX, or a combination of 800 mg WX and 400 mg VP per kg diet, respectively. Each diet was assigned to triplicate groups of fish in floating sea cages, and each cage was stocked with 60 fish. The results showed specific growth rate (SGR) and feed efficiency ratio (FER) were not significantly improved by the phytase supplementation. However, supplementations with WX and VP significantly enhanced the SGR and FER (P<0.05). Phosphorus retention was significantly increased by the supplementation with phytase, whereas nitrogen retention was also enhanced by supplementations of non-starch polysaccharide enzymes (P<0.05). The excretion experiment showed that the soluble phosphorus and ammonia nitrogen in starved fish were constant, irrespective of dietary treatments. The total phosphorus effluent in fish fed the diet with phytase supplementation was significantly lower compared with the control group after feeding to satiation (P < 0.05), but soluble phosphorus excretion was still independent of dietary treatments. The ammonia excretion in satiated fish significantly decreased with supplementations of non-starch polysaccharide enzymes. (C) 2007 Elsevier Inc. All rights reserved.
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