期刊
PURINERGIC SIGNALLING
卷 3, 期 3, 页码 203-219出版社
SPRINGER
DOI: 10.1007/s11302-006-9049-4
关键词
ecto-ATPase inhibitors; extracellular recordings; hippocampal slices; OGD; P2 purinergic receptors; synaptic potentials
资金
- University of Florence
- FIRB [RBAU019ZEN_004]
- PRIN-COFIN
- Ente Cassa Di Risparmio of Florence, Italy [2006/40600]
The role of ATP and its stable analogue ATP gamma S [adenosine-5'-o-(3-thio)triphosphate] was studied in rat hippocampal neurotransmission under normoxic conditions and during oxygen and glucose deprivation (OGD). Field excitatory postsynaptic potentials (fEPSPs) from the dendritic layer or population spikes (PSs) from the soma were extracellularly recorded in the CA1 area of the rat hippocampus. Exogenous application of ATP or ATP gamma S reduced fEPSP and PS amplitudes. In both cases the inhibitory effect was blocked by the selective A1 adenosine receptor antagonist DPCPX (8-cyclopentyl-1,3-dipropylxanthine) and was potentiated by different ecto-ATPase inhibitors: ARL 67156 (6-N,N-diethyl-D-beta,gamma-dibromomethylene), BGO 136 (1-hydroxynaphthalene-3,6-disulfonate) and PV4 [ hexapotassium dihydrogen monotitanoun-decatungstocobaltate(II) tridecahydrate, K6H2[TiW11CoO40] center dot 13H(2)O]. ATP gamma S-mediated inhibition was reduced by the P2 antagonist suramin [8-(3-benzamido-4-methylbenzamido) naphthalene-1,3,5-trisulfonate] at the somatic level and by other P2 blockers, PPADS (pyridoxalphosphate-6-azophenyl-2', 4'-disulfonate) and MRS 2179 (2'-deoxy-N-6-methyladenosine 3',5'-bisphosphate), at the dendritic level. After removal of both P2 agonists, a persistent increase in evoked synaptic responses was recorded both at the dendritic and somatic levels. This effect was prevented in the presence of different P2 antagonists. A 7-min OGD induced tissue anoxic depolarization and was invariably followed by irreversible loss of fEPSP. PPADS, suramin, MRS2179 or BBG (brilliant blue G) significantly prevented the irreversible failure of neurotransmission induced by 7-min OGD. Furthermore, in the presence of these P2 antagonists, the development of anoxic depolarization was blocked or significantly delayed. Our results indicate that P2 receptors modulate CA1 synaptic transmission under normoxic conditions by eliciting both inhibitory and excitatory effects. In the same brain region, P2 receptor stimulation plays a deleterious role during a severe OGD insult.
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