期刊
MODERN PATHOLOGY
卷 20, 期 6, 页码 656-667出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/modpathol.3800782
关键词
pancreatic intraepithelial neoplasia (PanINs); immunohistochemistry; precursor lesion; integrins; pancreatic cancer; chronic pancreatitis
类别
资金
- NCI NIH HHS [R21-CA102125, R21 CA102125, F31 CA106201] Funding Source: Medline
Pancreatic adenocarcinomas are highly invasive cancers for reasons that are currently unclear. Here we sought to determine if the proinvasive integrin alpha 6 beta 4 may be related to pancreatic adenocarcinoma tumor progression. Expression of integrin alpha 6 beta 4 was analyzed via immunohistochemistry for the beta 4 subunit in normal pancreas, pancreatic intraepithelial neoplasia (PanIN) lesions, pancreatic adenocarcinomas and chronic pancreatitis. In normal pancreatic ducts, integrin alpha 6 beta 4 was noted only at the cell's basal interface with the basement membrane. In pancreatic adenocarcinomas, 92% (104/113) demonstrated overexpression of integrin alpha 6 beta 4 and altered localization to the cytoplasm and membranous regions. This pattern of expression was observed in all PanIN lesions as early as PanIN-1A, and was evident in lesions that were juxtapositioned to normal epithelium. In contrast, 93% (13/14) of chronic pancreatitis samples resembled the staining pattern of normal pancreas. When cancer was present in areas of chronic pancreatitis, this altered expression of alpha 6 beta 4 integrin identified the cancer. We conclude that integrin alpha 6 beta 4 is expressed only on the basal surface of ductal cells in normal pancreas and chronic pancreatitis. During pancreatic adenocarcinoma progression, the alpha 6 beta 4 integrin is dramatically overexpressed and displays altered localization at the earliest stages of PanIN, thus representing an early event in pancreatic adenocarcinoma progression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据