Recent advances in aldolase-catalyzed asymmetric synthesis

This review is focused on advances over the last several years in the application of aldolases to organic synthesis. Several new technologies have been implemented to increase the scope and practicality of aldolases as tools for the synthetic chemist. These include directed evolution, discovery of new classes of aldolases in nature and the laboratory, and substrate and reaction engineering. 1 Introduction 2 Directed Evolution and Enzyme Engineering 2.1 DHAP-Dependent Aldolases 2.2 Pyruvate-Dependent Aldolases 2.3 Acetaldehyde-Dependent Aldoases 3, Discovery of Novel Aldolases 4 Reaction and Substrate Engineering 5 Future Prospects and Challenges