期刊
ANALYTICAL SCIENCES
卷 34, 期 10, 页码 1201-1208出版社
SPRINGERNATURE
DOI: 10.2116/analsci.18P281
关键词
Lipid oxidation; oxidized phospholipids; oxidative stress biomarkers; type 2 diabetes mellitus; zebrafish; LC-MS
资金
- Japan Society for the Promotion of Science
- Regional Innovation Strategy Support Program, Sapporo Health Innovation Smart-H, of the Ministry of Education, Culture, Sports, Science and Technology, Japan
- JSPS KAKENHI [25860294, 15K19074, 15KK0305]
- Grants-in-Aid for Scientific Research [15KK0305, 15K19074, 25860294] Funding Source: KAKEN
Type 2 diabetes mellitus is a serious metabolic disorder in the world. Oxidative stress, as a key role on the pathogenesis of diabetes, also results in the oxidation of phospholipids. However, studies on phospholipid oxidation in diabetes, especially directly focusing on oxidized and degraded phospholipid species, are quite limited. In this study, phospholipid profiles of diabetic zebrafish plasma were characterized by LC-HRMS and MS/MS, and the total amounts of each lipid class were compared. Furthermore, the key molecular species as biomarkers in distinguishing control and diabetic samples were investigated by orthogonal partial least squares discriminant analysis. Among the identified 114 phospholipid species in total, there were 11 hydroperoxides, 7 aldehydes, and 19 lysophospholipids found significantly elevated along with the increasing blood glucose, which were known as oxidation or degradation products. Furthermore, lysophosphatidylcholine 20:5 and lysophosphatidylcholine 22:6 were assessed as potential biomarkers in diabetic zebrafish. The current work would not only help to gain further insights into diabetes, but also contribute to find new clinical parameters for the screening of the promising antioxidant agents for its therapies.
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