期刊
ANALYTICAL SCIENCES
卷 34, 期 8, 页码 875-880出版社
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.17P462
关键词
Exosome; ion-exchange membrane; ion-depletion zone; ion-concentration polarization; concentration
资金
- JSPS KAKENHI [17H03246, 15K20989, 17K17715]
- JKA RING!RING! Project [27-183]
- Grants-in-Aid for Scientific Research [17H03246, 15K20989, 17K17715] Funding Source: KAKEN
Exosomes are of increasing research interest because they are integral to cell-cell communication and are implicated in various disease states. Here, we investigated the utility of using an ion-depletion zone in a microfluidic device to concentrate exosomes from the culture media of four types of cell lines. Furthermore, we evaluated the extent of damage to the exosomes following concentration by an ion-depletion zone microchannel device compared with exosomes concentrated by a conventional ultra-centrifugation technique. Our results conclusively demonstrate that significantly less damage is incurred by exosomes following passage through and concentration by the ion-depleted zone microchannel device compared to concentration by ultra-centrifugation. Our findings will help extend the utility of exosomes to various applications.
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