期刊
ANALYTICAL SCIENCES
卷 28, 期 3, 页码 295-299出版社
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.28.295
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资金
- AIST
- Grants-in-Aid for Scientific Research [22350040] Funding Source: KAKEN
In order to elucidate the role of desorption/ionization efficiency of peptides in MALDI-MS, we focused on peptides with disulfide bonds, which form a rigid tertiary structure. We synthesized seven sets of peptides with one disulfide bond (oxytocin, somatostatin, [Arg(8)]-vasopressin, [Arg(8)]-vasotocin, cortistatin, melanin-concentrating hormone, urotensin II-related peptide) and five sets of peptides with two disulfide bonds (tertiapin, alpha-conotoxin GI, alpha-conotoxin 1ml, a-conotoxin MI and alpha-conotoxin SI). Each peptide set consisted of three peptides: the oxidized form (S-S type), the reduced form (SH type), and an internal standard peptide in which all cysteine residues were substituted with alanine residues. In the case of urotensin II-related peptide, tertiapin, alpha-conotoxin ImI and alpha-conotoxin MI, the reduced form showed higher desorption/ionization efficiency than the oxidized form. In contrast, the other peptides revealed higher desorption/ionization efficiency in the oxidized form relative to the reduced form. These results imply that a rigid structure of peptides formed by disulfide bonds does not correlate with desorption/ionization efficiency in MALDI-MS.
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