Determination of Sulfide with Acidic Permanganate Chemiluminescence for Development of Deep-sea in-situ Analyzers

A new chemiluminescence method is proposed for the determination of sulfide in seawater based on the chemiluminescence reactior between sulfide and an acidic permanganate solution. 3-Cyclohexylaminopropanesulfonic acid was used as a chemiluminescence enhancer. By use of this method, 1 - 150 mu M of sulfide could be determined in artificial seawater. The limit of detection was 0.17 mu M sulfide. We investigated the effects of salinity, water temperature, and interfering chemicals such as heavy-metal ions and organic matter. In addition, natural seawater spiked with sulfide was analyzed. The results showed that the CL method could be applied to a deep-sea sulfide analyzer.