期刊
JOURNAL OF GENE MEDICINE
卷 9, 期 6, 页码 488-497出版社
WILEY
DOI: 10.1002/jgm.1041
关键词
non-viral gene therapy; genomic DNA; bacterial artificial chromosome (BAC); hydrodynamic
资金
- MRC [G9805886, G0400144, G0300705] Funding Source: UKRI
- Medical Research Council [G9805886, G0400144, G0300705] Funding Source: researchfish
- Medical Research Council [G9805886, G0300705, G0400144] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
Background The delivery of a complete genomic DNA locus in vivo may prove advantageous for complementation gene therapy, especially when physiological regulation of gene expression is desirable. Hydrodynamic tail vein injection has been shown to be a highly efficient means of non-viral delivery of plasmid DNA to the liver. Here, we apply hydrodynamic tail vein injection to deliver and express large genomic DNA inserts >100 kb in vivo. Methods Firstly, a size series (12-172 kb) of bacterial artificial chromosome (BAC) plasmids, carrying human genomic DNA inserts, episomal retention elements, and the enhanced green fluorescent protein (EGFP) reporter gene, was delivered to mice by hydrodynamic tail vein injection. Secondly, an episomal BAC vector carrying the whole genomic DNA locus of the human low-density lipoprotein receptor (LDLR) gene, and an expression cassette for was delivered by the same method. the LacZ reporter gene. Results We show that the efficiency of delivery is independent of vector size, when an equal number of plasmid molecules are used. We also show, by LacZ reporter gene analysis, that BAC delivery within the liver is widespread. Finally, BAC-end PCR, RT-PCR and immunchistochemistry demonstrate plasmid retention and long-term expression (4 months) of human LDLR in transfected hepatocytes. Conclusion This is the first demonstration of somatic delivery and longterm expression of a genomic DNA transgene >100 kb in vivo and shows that hydrodynamic tail vein injection can be used to deliver and express large genomic DNA transgenes in the liver. Copyright (C) 2007 John Wiley & Sons, Ltd.
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