期刊
ANALYTICAL SCIENCES
卷 25, 期 3, 页码 339-346出版社
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.25.339
关键词
-
资金
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan [17655059, 19350045, 20043011, 20050007]
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
In this paper, we describe systematic detailed considerations of the feasibility of using various metal nanoparticles for organic-matrix-free surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). In order to avoid the influence of organic molecules on the nanoparticles, stabilizer-free bare nanoparticles of Ag, Au, Cu and Pt were prepared by laser ablation. Although all metal nanoparticles absorbed N-2 laser light (337 nm) energy, the performance of desorption/ionization of a representative peptide, angiotensin 1, strongly depended on the metal element. Citrate buffer was used as a proton source; it reduced the amount of alkali cation adducts present. Then, protonated molecules of analytes predominated in the mass spectra when Au and Pt nanoparticles were used. Pt nanoparticles showed the highest performance in SALDI-MS, owing to their smaller heat conductivity and higher melting temperature. The selective desorption of a cationic surfactant with longer alkyl chains and a peptide with methionine was also observed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据