期刊
JOURNAL OF IMMUNOLOGY
卷 178, 期 11, 页码 6806-6813出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.178.11.6806
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- NHLBI NIH HHS [R01 HL31809] Funding Source: Medline
Sphingosine 1-phosphate (SIP) in blood and lymph controls lymphoid traffic and tissue migration of T cells through signals from the type 1 S1PR (S1P(1)), but less is known of effects of the SIP-S1P, axis on nonmigration functions of T cells. CD4 T cells from a double transgenic (DTG) mouse express OTII TCRs specific for OVA peptide 323-339 (OVA) and a high level of transgenic Sip,, resistant to suppression by T cell activation. OVA-activated DTG CD4 T cells respond as expected to Sip by chemotactic migration and reduction in secretion of IFN-gamma. In addition, DTG CD4 T cells stimulated by OVA secrete a mean of 2.5-fold more IL-17 than those from OTII single transgenic mice with concomitantly higher levels of mRNA encoding IL-17 by real-time PCR and of CD4 T cells with intracellular IL-17 detected by ELISPOT assays. OVA challenge of s.c. air pockets elicited influx of more OTII TCR-positive T cells producing a higher level of IL-17 in DTG mice than OTII control mice. Augmentation of the number and activity of Th17 cells by the S1P-S1P(1) axis may thus enhance host defense against microbes and in other settings increase host susceptibility to autoimmune diseases.
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