期刊
ANALYTICAL SCIENCES
卷 25, 期 3, 页码 347-352出版社
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.25.347
关键词
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资金
- 973 National Key Basic Research Program [2007CB310500]
- National NSF of China [20435010, 20575020, 20675028, 20605007, 20775023]
- Ministry of Education [NCET-04-0768]
We have reported on a novel enzyme immunoassay method for the detection of protein using biocatalytic silver nanoparticles as an enhanced substrate based on surface-enhanced Raman scattering (SERS). First, ascorbic acid was converted front ascorbic acid 2-phosphate by alkaline phosphatase immobilized on polystyrene microwells after a typical sandwich immunoreaction. Then Ag(I) ions were reduced to silver nanoparticles by the obtained ascorbic acid, which would result in a SERS signal when Raman dyes were absorbed. Using human IgG as a model protein, a wide linear dynamic range (1 to 100 ng ml(-1)) was reached with a low detection limit (0.02 ng ml-1) under the optimized assay conditions. Moreover, the production of an enhanced substrate was chosen as the signaling element in this method, which demonstrates a new way for SERS-based quantitative detection. These results suggest that the application of SERS enhanced by biocatalytic production of metal nanopaticles holds a promising potential for a sensitive immunoassay.
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