期刊
ANALYTICAL METHODS
卷 10, 期 36, 页码 4430-4437出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8ay00736e
关键词
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资金
- National Natural Science Foundation of China [21675109, 21475084]
- China Postdoctoral Science Foundation [2016M590684]
- Program for Science & Technology Innovation Talents in Universities of Henan Province [16HASTIT005]
- Natural Science Foundation of Henan Province [162300410209]
- Innovation Scientists and Technicians Troop Construction Projects of Henan Province [41]
- Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases
In this study, we developed a novel and facile electrochemical aptasensor for beta-amyloid (A beta) oligomer detection based on metal-organic frameworks (MOFs) as signal probes. By using an aptamer-tethered gold nanoflower (AuNF) modified electrode for target capture and aptamer-tagged gold nanoparticles/Cu-MOF (AuNPs/Cu-MOF) conjugates for sensitive signal generation, an effective sandwich sensor was obtained for the detection of A beta oligomers. The unique physico-chemical properties of AuNPs/Cu-MOF conjugates, such as high electrochemical activity and large surface area, endow the sensor with excellent analytical performances. The designed aptasensor exhibits a wide linear range from 1 nM to 2 mu M with a linear correlation coefficient of 0.996 and a relatively low detection limit of 0.45 nM for the detection of A beta oligomers. For the evaluation of A beta oligomers in artificial cerebrospinal fluid, the high recoveries and accuracy indicate the feasibility, which will provide valuable information for the early diagnosis of Alzheimer's disease. Moreover, the proposed electrochemical aptasensor based on multifunctional MOFs will provide a general strategy for sensing other analytes.
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