期刊
MOLECULAR ONCOLOGY
卷 1, 期 1, 页码 84-96出版社
WILEY
DOI: 10.1016/j.molonc.2007.02.004
关键词
Breast cancer; 3D culture; Extracellular matrix; Gene expression profiling; Signal transduction
类别
资金
- Department of Defense Breast Cancer [DAMD17-02-1-0438, DAMD17-00-1-0224]
- US Department of Energy, Office of Biological and Environmental Research [DE-AC03SF0098]
- National Cancer Institute [R01 CA064786, U54 CA112970]
- Susan G. Komen Breast Cancer Foundation [2000-223]
3D cell cultures are rapidly becoming the method of choice for the physiologically relevant modeling of many aspects of non-malignant and malignant cell behavior ex vivo. Nevertheless, only a limited number of distinct cell types have been evaluated in this assay to date. Here we report the first large scale comparison of the transcriptional profiles and 3D cell culture phenotypes of a substantial panel of human breast cancer cell lines. Each cell line adopts a colony morphology of one of four main classes in 3D culture. These morphologies reflect, at least in part, the underlying gene expression profile and protein expression patterns of the cell lines, and distinct morphologies were also associated with tumor cell invasiveness and with cell lines originating from metastases. We further demonstrate that consistent differences in genes encoding signal transduction proteins emerge when even tumor cells are cultured in 3D microenvironments. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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