期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 388, 期 4, 页码 871-879出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-007-1296-x
关键词
pro-oxidant; antioxidant; ROS; bioactivation; on-line; biochemical detection
资金
- ATSDR CDC HHS [E11 TS000091] Funding Source: Medline
Reactive oxygen species (ROS) can damage proteins, cause lipid peroxidation, and react with DNA, ultimately resulting in harmful effects. Antioxidants constitute one of the defense systems used to neutralize pro-oxidants. Since pro-oxidants and antioxidants are found ubiquitously in nature, pro-and antioxidant effects of individual compounds and of mixtures receive much attention in scientific research. A major bottleneck in these studies, however, is the identification of the individual pro-oxidants and antioxidants in mixtures. Here, we describe the development and validation of an on-line post-column biochemical detection system for ROS-producing compounds and antioxidants in mixtures. Inclusion of cytochrome P450s and cytochrome P450 reductase also permitted the screening of compounds that need bioactivation to exert their ROS-producing properties. This pro-oxidant and antioxidant detection system was integrated on-line with gradient HPLC. The resulting high-resolution screening technology was able to separate mixtures of ROS-producing compounds and antioxidants, allowing each species to be characterized rapidly and sensitively.
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