期刊
ANALYTICAL METHODS
卷 6, 期 17, 页码 7006-7014出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ay01056f
关键词
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资金
- Program of Guizhou Provincial Tobacco Company [201112, 201431]
- Key Special Program of China National Tobacco Corporation [TS-02-20110014]
- Foundation of Science and Technology of Guizhou Province of China [J[2013]2195]
To evaluate the glycoconjugated aroma compounds of three cultivars of tobacco, an enzymatic hydrolysis method followed by gas chromatography-mass spectroscopy (GC-MS) was successfully established using a series optimization approach. In the first step, two hydrolytic enzymes were compared and beta-glucosidase was chosen. Then, the critical variables that affect the concentration of aglycons were searched by Plackett-Burman design (PBD) and further optimized by central composite design (CCD) of response surface methodology. The optimum conditions were: 60 h for hydrolysis time, 36.8 degrees C for hydrolysis temperature, 5.6 for pH, 2.1 mL for solvent volume, 2 mg mL(-1) for beta-glucosidase concentration and 200 rpm for agitation speed. Under the optimized experimental conditions, this method gave a satisfactory precision and linearity with relative standard deviation (RSD) less than 20% and Pearson correlation coefficients greater than 0.99. Finally, the proposed method was successfully employed to analyze flue-cured tobacco (FCT), sun-cured tobacco (SCT) and oriental tobacco (OT). Most of the aglycons were significantly different in the three cultivars and the corresponding characteristic components were discussed in detail.
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